The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

@article{Taylor1985TheRG,
  title={The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.},
  author={John W Taylor and J. Ott and Fritz Eckstein},
  journal={Nucleic acids research},
  year={1985},
  volume={13 24},
  pages={
          8765-85
        }
}
M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)strand. The reaction of Pvu I with M13mp2 RF IV DNA containing dCMPS residues in the (-)strand is of this type, and is exploited to allow subsequent digestion with exonuclease III of a portion of the… CONTINUE READING

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