The rapid discovery and identification of physalins in the calyx of Physalis alkekengi L.var.franchetii (Mast.) Makino using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry together with a novel three-step data mining strategy.

@article{Huang2014TheRD,
  title={The rapid discovery and identification of physalins in the calyx of Physalis alkekengi L.var.franchetii (Mast.) Makino using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry together with a novel three-step data mining strategy.},
  author={Cai Huang and Qiongming Xu and Chang Chen and Chengwu Song and Y. Xu and Yi Xiang and Y. Feng and H. Ouyang and Y. Zhang and Hongliang Jiang},
  journal={Journal of chromatography. A},
  year={2014},
  volume={1361},
  pages={
          139-52
        }
}
Physalins, uniquely discovered from genus physalis, showed significant bioactivities in many aspects. It is therefore very important for the exploration of natural resources rich of physalins. However, there is no efficient approach for rapid discovery and identification of this class of compounds due to their structural complexity. To address the issue, the fragmentation pathways and correspondingly fragmentation rules of physalins in negative MS/MS mode were thoroughly investigated in this… Expand
Metabolic profiles of physalin A in rats using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
TLDR
A rapid and reliable ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS) method was developed and employed for the comprehensive study of the metabolism of physalin A in vivo for the first time and indicated that sulfonation, reduction and hydroxylation were the major metabolic pathways of Physalis alkekengi var. Expand
High performance thin-layer chromatography-mass spectrometry enables reliable analysis of physalins in different plant parts of Physalis alkekengi L.
We developed first HPTLC and HPTLC-MS/MS methods which enable characterization of structurally similar and complex biologically active compounds - physalins - from crude extracts of Chinese lanternExpand
Non-targeted chromatographic analyses of cuticular wax flavonoids from Physalis alkekengi L.
TLDR
A non-targeted approach for the separation, isolation and identification of methylated flavonoids present in P. alkekengi L. franchetii cuticular waxes was established and a rationale for the mechanism of separation by HPLC is given, which establishes a foundation for future development of chromatographic methods for methylated Flavonols and related compounds. Expand
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TLDR
This study increases knowledge of the chemical composition of the calyces of this fruit and thereby supports the recycling of this class of residue, which could be used as phytotherapeutic, nutraceutic, or cosmetic ingredients for the development of diverse natural products. Expand
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Isolation and characterization of cytotoxic withanolides from the calyx of Physalis alkekengi L. var franchetii.
TLDR
Phytochemical investigation into the calyx of Physalis alkekengi L. var franchetii (Mast) Makino resulted in the isolation of ten cytotoxic withanolide, including five new withanolides, 1-5, which were elucidated by extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. Expand
LC-MS/MS method for simultaneous determination of flavonoids and physalins in rat plasma: Application to pharmacokinetic study after oral administration of Physalis alkekengi var. franchetii (Chinese lantern) extract.
TLDR
A rapid and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed and validated and successfully applied for the first time to the pharmacokinetic study of five ingredients after oral administration of 70% ethanol extract of Chinese lantern in rats. Expand
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TLDR
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The KuDieZi (KDZ) injection is prepared by extracting and processing Ixeris sonchifolia [Ixeris sonchifolia Hance] belonging to Lactuca genus of the Asteraceae family. The KDZ injection is aExpand
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References

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Characterization of physalins and fingerprint analysis for the quality evaluation of Physalis alkekengi L. var. franchetii by ultra-performance liquid chromatography combined with diode array detection and electrospray ionization tandem mass spectrometry.
TLDR
The proposed method provides a scientific and technical platform to the herbal industry for quality control and safety assurance of herbal preparations that contain this class of physalins. Expand
Rapid identification of ophiopogonins and ophiopogonones in Ophiopogon japonicus extract with a practical technique of mass defect filtering based on high resolution mass spectrometry.
TLDR
The present results of rapid detection and identification of ophiopogonins and ophypogonones suggest that the proposed MDF approach based on the high-resolution mass spectrometry data would be expected adaptable to the analysis of other herbal components. Expand
Characterization and identification of steroidal alkaloids in Fritillaria species using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
TLDR
It was found that hydrogen rearrangement and induction effects result in ring cleavage of the basic skeletons occurred in the MS/MS process and produced characteristic fragment ions, which are useful for structural elucidation. Expand
A practical strategy for the characterization of coumarins in Radix Glehniae by liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry.
TLDR
A practical method for the characterization of coumarins in Radix Glehniae by liquid chromatography-mass spectrometry (LC-MS), the first time that these two scan modes have been combined to characterize chemical constituents in traditional Chinese medicine. Expand
Antimycobacterial physalins from Physalis angulata L. (Solanaceae)
TLDR
Structural elucidation of both physalins D and B was based on detailed 13C and 1H NMR spectral analysis with the aid of 2D‐correlation spectroscopy, and the assignment of the 13C chemical shift for physalin D is reported here for the first time. Expand
Identification of regioisomers of methylated kaempferol and quercetin by ultra high performance liquid chromatography quadrupole time-of-flight (UHPLC-QTOF) tandem mass spectrometry combined with diagnostic fragmentation pattern analysis.
TLDR
The regioisomers of methylated kaempferol and quercetin are examined using ultra high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry for characterising the specificity of novel flavonoid O-methyltransferases and can help direct enzymatic or chemical syntheses during the early stages of drug discovery. Expand
Immune depression in Rhodnius prolixus by seco-steroids, physalins.
TLDR
Results suggest that, in different ways, probably due to their different chemical structures, physalin B, D, F and G are immunomodulatory substances for the bloodsucking insect, R. prolixus. Expand
Diagnostic fragment-ion-based extension strategy for rapid screening and identification of serial components of homologous families contained in traditional Chinese medicine prescription using high-resolution LC-ESI- IT-TOF/MS: Shengmai injection as an example.
The paper presents a modified and universally applicable diagnostic fragment-ion-based extension strategy (DFIBES) to efficiently process the information acquired by liquidExpand
Physalin and neophysalins from Physalis alkekengi var. francheti and their differentiation inducing activity
A methanol extract of Physalis alkekengi var. francheti showed a potent cell differentiation inducing activity toward mouse myeloid leukemia cell line (M1 cells). From the extract, a new physalin andExpand
Steroids and flavonoids from Physalis alkekengi var. franchetii and their inhibitory effects on nitric oxide production.
TLDR
Four new steroidal compounds possessing a rare 13,14-seco-16,24-cycloergostane skeleton, Physalis alkekengi var. Expand
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