The radiometric assay of microsomal UDP-glucuronyltransferase using 125I-labelled phenolphthalein.

  • Swee Eng Aw
  • Published 1975 in
    Clinica chimica acta; international journal of…

Abstract

A rapid and sensitive method is described for assaying UDP-glucuronyl-transferase (EC 2.4.1.17) activity. The substrate is the relatively apolar phenolphthalein, labelled with 125I on one of its two phenolic rings leaving a free hydroxyl group for glucuronidation. Extraction with ethyl acetate leaves the glucuronide in the aqueous reaction medium which is… (More)

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