The quantification of prion gene expression in sheep using real-time RT-PCR

@article{Han2006TheQO,
  title={The quantification of prion gene expression in sheep using real-time RT-PCR},
  author={Cai-Xia Han and Hong-Xiang Liu and De-Ming Zhao},
  journal={Virus Genes},
  year={2006},
  volume={33},
  pages={359-364}
}
Determination of the transcription level of cellular prion protein (PrPC) is essential for understanding its poorly explained role in organisms. Scrapie in sheep is the prototype of all prion diseases. However, the expression of prion protein (PrP) mRNA in sheep has not been quantified in great detail. Herein we report on measurement of sheep PrP mRNA using absolute quantitative real-time reverse transcription and polymerase chain reaction (RT-PCR). Total RNA was isolated from seven different… CONTINUE READING

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Quantification of PrP mRNA in sheep tissues Tissues No. of samples Total RNA (ng/mg tissue) CV% PrP mRNA (copies/ng total RNA) CV% CNS Obex

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