The pyruvate:ferredoxin oxidoreductase enzyme is located in the plasma membrane and in a cytoplasmic structure in Entamoeba.

@article{Rodrguez1998ThePO,
  title={The pyruvate:ferredoxin oxidoreductase enzyme is located in the plasma membrane and in a cytoplasmic structure in Entamoeba.},
  author={Mario Alberto Rodr{\'i}guez and Rosa Mar{\'i}a Garc{\'i}a-P{\'e}rez and L Romero Mendoza and Tomas Clemente Sanchez and Nancy Guillen and Esther Orozco},
  journal={Microbial pathogenesis},
  year={1998},
  volume={25 1},
  pages={1-10}
}
This work investigated the cellular location of the pyruvate:ferredoxin oxidoreductase (PFO) enzyme in Entamoeba. A 1.9 kb fragment located at the 3' end of the Ehpfo gene was cloned in the pRSETB vector and expressed. The recombinant peptide was purified and inoculated in rabbits. By Western blot assays the antibodies detected a single 130 kDa band in all E. histolytica strains tested and in E. moshkovskii. By immunofluorescence, the antibodies showed the presence of PFO in the plasma membrane… CONTINUE READING

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