The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3

  title={The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3},
  author={Paul A. C. Cloos and Jesper Heile Christensen and Karl Agger and Alessio Maiolica and Juri Rappsilber and Torben L Antal and Klaus H. Hansen and Kristian Helin},
Methylation of lysine and arginine residues on histone tails affects chromatin structure and gene transcription. Tri- and dimethylation of lysine 9 on histone H3 (H3K9me3/me2) is required for the binding of the repressive protein HP1 and is associated with heterochromatin formation and transcriptional repression in a variety of species. H3K9me3 has long been regarded as a ‘permanent’ epigenetic mark. In a search for proteins and complexes interacting with H3K9me3, we identified the protein… 

Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins*S⃞

It is reported that the euchromatic histone lysine methyltransferase G9a/KMT1C mediates H1.4K26 mono- and dimethylation in vitro and in vivo and thereby provides a recognition surface for the chromatin-binding proteins HP1 and L3MBTL1.

UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development

It is shown that the human JmjC-domain-containing proteins UTX and JMJD3 demethylate tri-methylated Lys 27 on histone H3, and the results suggest that H3K27me3 dem methylation regulated by UTX/JMJD3 proteins is essential for proper development.

Demethylation of Histone H3K36 and H3K9 by Rph1: a Vestige of an H3K9 Methylation System in Saccharomyces cerevisiae?

The capacity of Rph1 to demethylate H3K9 provides the first indication that S. cerevisiae may have once encoded an H3k9 methylation system and suggests that Rph 1 is a functional vestige of this modification system.

The Histone Demethylases JMJD1A and JMJD2B Are Transcriptional Targets of Hypoxia-inducible Factor HIF*

It is shown that the hypoxia-inducible factor HIF-1α binds to specific recognition sites in the genes encoding the jumonji family histone demethylases JMJD1A and JMJD2B and induces their expression and increased expression in renal cancer cells that have lost the von Hippel Lindau tumor suppressor protein VHL.

The Saccharomyces cerevisiae Histone Demethylase Jhd1 Fine-Tunes the Distribution of H3K36me2

These studies establish JHD1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units.

ICBP90, a Novel Methyl K9 H3 Binding Protein Linking Protein Ubiquitination with Heterochromatin Formation

Using an unbiased in vitro biochemical approach, ICBP90 is identified as a novel methyl K9 H3-specific binding protein that depends on a PHD finger that defines the binding specificity and an SRA domain that promotes binding activity.

JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer

JARID1B is identified as a demethylase capable of removing three methyl groups from histone H3 lysine 4 and up-regulated in prostate cancer and associates with androgen receptor and regulates its transcriptional activity.



Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins

It is shown that mammalian methyltransferases that selectively methylate histone H3 on lysine 9 (Suv39h HMTases) generate a binding site for HP1 proteins—a family of heterochromatic adaptor molecules implicated in both gene silencing and supra-nucleosomal chromatin structure.

Histone demethylation by a family of JmjC domain-containing proteins

The JmjC domain is identified as a novel demethylase signature motif and a protein demethylation mechanism that is conserved from yeast to human is uncovered.

Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain

A stepwise model for the formation of a transcriptionally silent heterochromatin is provided: SUV39H1 places a ‘methyl marker’ on histone H3, which is then recognized by HP1 through its chromo domain, which may also explain the stable inheritance of theheterochromatic state.

Methylation: lost in hydroxylation?

It is proposed that the fission yeast protein Epe1 is a putative histone demethylase that could act by oxidative demethylation, and certain other chromatin‐associated JmjC‐domain proteins may be protein hydroxylases that catalyse a novel histone modification.

The role of heterochromatin in centromere function

  • A. PidouxR. Allshire
  • Biology
    Philosophical Transactions of the Royal Society B: Biological Sciences
  • 2005
An epigenetic model for ensuring that CENP-A is targeted and replenished at the kinetochore domain is discussed, which could have additional roles in centromere architecture and the prevention of merotely.

Structure of Human FIH-1 Reveals a Unique Active Site Pocket and Interaction Sites for HIF-1 and von Hippel-Lindau*

The crystal structure of human FIH-1 is reported, revealing the unique environment of the active site and cofactor-binding region revealed in the structure that should allow design of selective drugs that can be used in ischemic diseases to promote hypoxia responses.

Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines.

The findings suggest that overexpressed GASC1 may play an important role in the development and/or progression of various types of cancer including ESC.