The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis by colloidal silica (Percoll) gradient centrifugation.

Abstract

Percoll gradient centrifugation has been applied to Plasmodium falciparum- and P. yoelii-infected blood to allow rapid concentration of gametocytes. In the case of cultured P. falciparum, stage IV and V gametocytes were separated from immature stage II and III forms and concentrated from initial gametocytaemias of 1 to 3% to an average of greater than 80… (More)

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