The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells.

@article{Neddermann1993ThePO,
  title={The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells.},
  author={Petra Neddermann and Josef Jiricny},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 28},
  pages={
          21218-24
        }
}
G/T mispairs that arise in the DNA of higher eukaryotes as a result of spontaneous hydrolytic deamination of 5-methylcytosine to thymine must be corrected to G/C pairs. We describe here the purification to apparent homogeneity of the enzyme that initiates this repair process by excising the mispaired thymine from the hetero-duplex to generate an apyrimidinic site. The enzymatic activity could be attributed to a 55-kDa polypeptide, which was purified from extracts of HeLa cells by a combination… CONTINUE READING

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