The purification and spectral properties of polyphenol oxidase I fromNicotiana tabacum

@article{Shi2007ThePA,
  title={The purification and spectral properties of polyphenol oxidase I fromNicotiana tabacum},
  author={Chunhua Shi and Ya Nan Dai and Bingle Xia and Xiaolong Xu and Yongshu Xie and Qingliang Liu},
  journal={Plant Molecular Biology Reporter},
  year={2007},
  volume={19},
  pages={381-382}
}
Polyphenol oxidase plays a key role in plant defense systems. We report the first-time purification of polyphenol oxidase (PPO 1.14.18.1) from fresh leaves of tobacco (Nicotiana tabacum) using acetone powder, ammonium sulfate precipitation, and column chromatography with DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-75. PPO I was purified approximately 71-fold (3200 U/mg). The MALDI-TOF-MS spectrum showed that the enzyme was purified to a pure protein with a molecular weight of 35700 Da… CONTINUE READING

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Max Wellington

2007
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References

Publications referenced by this paper.
Showing 1-10 of 22 references

Mechanism of browning in fresh highbush blueberry fruit, partial purification and characterization of blueberry polyphenol oxidase

F Kader, B Rovel, M Girardin, M Metche
J Sci Food Agric • 1997
View 3 Excerpts
Highly Influenced

Fractionation and identification of the phenolic compounds of highbush blueberry

F Kader, B Rovel, M Girardin, M Metche
Food Chem • 1996
View 2 Excerpts
Highly Influenced

Copper Protein and Copper enzyme

DA Robb
Vol 2. In: Contie R (ed). pp 207241 • 1984
View 2 Excerpts
Highly Influenced

Purification and properties of polyphenol oxidase from loquat fruit

CK Ding, K Chachin, Y Ueda
J Agric Food Chem • 1998

Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit

JR Das, SG Bhat, LR Gowda
J Agric Food Chem • 1997

Isolation and characterization of spinach photosystem II membrane-associated catalase and polyphenol oxidase

G Yelena, Sheptovitsky, GW Brudvig
Biochemistry • 1996
View 1 Excerpt

Systemic wound induction of potato (Solanum tuberosum) polyphenol oxidase

P Thipyapong, DM Joel, JC Steffens
Phytochemistry • 1995
View 1 Excerpt