The purification and spectral properties of polyphenol oxidase I fromNicotiana tabacum

  title={The purification and spectral properties of polyphenol oxidase I fromNicotiana tabacum},
  author={Chunhua Shi and Ya Nan Dai and Bingle Xia and Xiaolong Xu and Yongshu Xie and Qingliang Liu},
  journal={Plant Molecular Biology Reporter},
Polyphenol oxidase plays a key role in plant defense systems. We report the first-time purification of polyphenol oxidase (PPO from fresh leaves of tobacco (Nicotiana tabacum) using acetone powder, ammonium sulfate precipitation, and column chromatography with DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-75. PPO I was purified approximately 71-fold (3200 U/mg). The MALDI-TOF-MS spectrum showed that the enzyme was purified to a pure protein with a molecular weight of 35700 Da… CONTINUE READING


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