The purification and some properties of H-lysin from Aeromonas salmonicida.

@article{Titball1985ThePA,
  title={The purification and some properties of H-lysin from Aeromonas salmonicida.},
  author={Richard W. Titball and Colin Bernhard Munn},
  journal={Journal of general microbiology},
  year={1985},
  volume={131 7},
  pages={1603-9}
}
H-lysin from Aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. The purified material was predominantly H-lysin, devoid of detectable T-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (GCAT) activity was present. The results suggested that H-lysin and GCAT activities were due to different extracellular products. Studies of the… CONTINUE READING