The purification and protective capacity of Bordetella pertussis outer membrane proteins.

Abstract

The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-associated 92, 32 and 30 kDa OMPs were purified and the N-terminal amino acid sequences were determined. The N-terminal amino acid sequences of the 30 and 32 kDa OMPs show homology with the C-terminal fragment of the P.93 precursor of the 69 kDa OMP (pertactin). The purified 32 kDa OMP was protective in the i.c. test when presented as mixed protein-detergent micelles. The 92 kDa OMP became a protective antigen when nonprotective levels of pertussis toxin were added. We found a correlation between the i.c. mouse protection value and the 92 kDal38 kDa (porin) ratio in OMC preparations.

Cite this paper

@article{Hamstra1995ThePA, title={The purification and protective capacity of Bordetella pertussis outer membrane proteins.}, author={Hendrik Jan Hamstra and Betsy Kuipers and D Schijf-Evers and H G Loggen and Jan T. Poolman}, journal={Vaccine}, year={1995}, volume={13 8}, pages={747-52} }