The purification and polypeptide composition of avian infectious bronchitis virus.

@article{Alexander1977ThePA,
  title={The purification and polypeptide composition of avian infectious bronchitis virus.},
  author={Dennis J. Alexander and Margaret S. Collins},
  journal={Microbios},
  year={1977},
  volume={18 72},
  pages={87-98}
}
Purification of egg-grown infectious bronchitis virus (IBV) by sucrose density gradient centrifugation alone, or sucrose density gradient centrifugation plus pH 8.0 treatment, concanavalin A precipitation or metrizamide density gradient centrifugation, failed to produce any differences in the virus polypeptide pattern following polyacrylamide gel electrophoresis in the presence of SDS(SDS-PAGE). SDS-PAGE of purified IBV on 7.5% acrylamide gels separated 16 polypeptides which were detectable by… CONTINUE READING