The purification and characterization of radiolabelled parathyroid hormone for radioimmunoassay.

@article{Slling1982ThePA,
  title={The purification and characterization of radiolabelled parathyroid hormone for radioimmunoassay.},
  author={H S\olling},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1982},
  volume={122 1},
  pages={11-9}
}
  • H Sølling
  • Published 1982 in
    Clinica chimica acta; international journal of…
An improved method for the purification of 125I-labelled PTH is presented. Following iodination of the hormone by the chloramine-T method, the reaction mixture is transferred directly to a 0.9 x 9 cm column of carboxymethyl-cellulose. Two major peaks emerge with the ammonium acetate gradient. Peak I represents labelled intact bPTH, Mr 9600, of good quality, i.e. a high B0 and a low NSB in the radioimmunoassay. Peak II, Mr approximately 12000-18000, gives a high B0, but also a high NSB and is… CONTINUE READING