The proteomes of transcription factories containing RNA polymerases I, II or III

@inproceedings{Melnik2011ThePO,
  title={The proteomes of transcription factories containing RNA polymerases I, II or III},
  author={Svitlana Melnik and Binwei Deng and Argyris Papantonis and Sabyasachi Baboo and Ian M. Carr and Peter R. Cook},
  booktitle={Nature Methods},
  year={2011}
}
Human nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammalian cells. After isolation, we analyzed their protein content by mass spectrometry. Each complex represents part of the core of a transcription factory. For example, the RNA polymerase II complex… CONTINUE READING
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