The proteome buccaneers: how to unearth your treasure chest via combinatorial peptide ligand libraries.

@article{Righetti2010ThePB,
  title={The proteome buccaneers: how to unearth your treasure chest via combinatorial peptide ligand libraries.},
  author={Pier Giorgio Righetti and Egisto Boschetti and Alexander V Kravchuk and Elisa Fasoli},
  journal={Expert review of proteomics},
  year={2010},
  volume={7 3},
  pages={373-85}
}
The latest advances in combinatorial peptide ligand libraries, with their unique performance in discovering low-abundance species in proteomes, are reviewed here. Explanations of mechanism, potential applications, capture of proteomes at different pH values to enhance the total catch and quantitative elutions, such as boiling in the presence of 5% sodium dodecyl sulfate and 3% dithiothreitol are included. The reproducibility of protein capture among different experiments with the same batch of… CONTINUE READING

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