The protein cofactor allows the sequence of an RNase P ribozyme to diversify by maintaining the catalytically active structure of the enzyme.

@article{Kim1997ThePC,
  title={The protein cofactor allows the sequence of an RNase P ribozyme to diversify by maintaining the catalytically active structure of the enzyme.},
  author={Jaeyoun Kim and Ahmed F Kilani and Xi Zhan and S. Altman and Fenyong Liu},
  journal={RNA},
  year={1997},
  volume={3 6},
  pages={613-23}
}
To study the effect proteins have on the catalysis and evolution of RNA enzymes, we simulated evolution of RNase P catalytic M1 RNA in vitro, in the presence and absence of its C5 protein cofactor. In the presence of C5, functional M1 sequence variants (not catalytically active in the absence of C5) were selected in addition to those identical to M1. C5 maintains the catalytically active structure of the variants and allows for an enhanced spectrum of M1 molecules to function in the context of… CONTINUE READING