The protein binding of acetylsalicylic acid and salicylic acid.

  title={The protein binding of acetylsalicylic acid and salicylic acid.},
  author={M. Asgar Ali and Joseph Isaac Routh},
  journal={Clinical chemistry},
  volume={15 11},
Quantitative experiments to study the binding of acetylsalicylic acid (ASA) and salicylic acid (SA) by human serum albumin (HSA) were carried out using 14C-labeled ASA or SA and gel filtration to separate the free from the bound forms. Two binding procedures were employed: the ASA or SA was incubated with the HSA, or the mixture was placed in an equilibrium dialysis cell. By withdrawing samples at intervals, the extent of binding or the attainment of equilibrium could be assessed. Evidence that… 

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  • J. Pharnuacol. Eu-p. Therap. 21,
  • 1923

The syuutluesis of acetyl1 - C 14salicylic acid

  • J . Amer . Pharm . A 88 . ( Sci . Ed . )
  • 1951

A micro method for analysis of phasuuua salicyhate

  • Proc . Soc . Exp . Biol . Med .
  • 1964