The promoter of the latency-associated transcripts of herpes simplex virus type 1 contains a functional cAMP-response element: role of the latency-associated transcripts and cAMP in reactivation of viral latency.

@article{Leib1991ThePO,
  title={The promoter of the latency-associated transcripts of herpes simplex virus type 1 contains a functional cAMP-response element: role of the latency-associated transcripts and cAMP in reactivation of viral latency.},
  author={David A. Leib and Kari Christine Nadeau and Shelley A. Rundle and Priscilla A. Schaffer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 1},
  pages={48-52}
}
A 203-base-pair sequence 5' of the latency-associated transcripts (LATs) of herpes simplex virus type 1 contains a 7-base consensus sequence TGCGTCA that is identical to the cAMP-response element of the proenkephalin gene. This consensus sequence is at -38 relative to the putative 5' end of the LATs with a TATA box at the -24 position. In transient chloramphenicol acetyltransferase assays in rat pheochromocytoma (PC12) cells, this enhancer region stimulated gene expression up to 3-fold in the… CONTINUE READING

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In transient chloramphenicol acetyltransferase assays in rat pheochromocytoma ( PC12 ) cells , this enhancer region stimulated gene expression up to 3-fold in the presence of dibutyryl cAMP , forskolin , nerve growth factor , or phorbol 12-myristate 13-acetate .
In transient chloramphenicol acetyltransferase assays in rat pheochromocytoma ( PC12 ) cells , this enhancer region stimulated gene expression up to 3-fold in the presence of dibutyryl cAMP , forskolin , nerve growth factor , or phorbol 12-myristate 13-acetate .
These studies demonstrate that the promoter region of the LATs contains a functional cAMP - response element and that expression of the LATs is likely controlled by second messenger signal transduction and imply a role for cAMP in triggering viral reactivation .
However , these reagents did not accelerate reactivation of a deletion mutant that lacks the putative cAMP - response element - containing promoter region , transcriptional start site , and 1015 base pairs of the LATs .
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