The promoter of the Immunoglobulin J Chain gene receives its authentic enhancer activity through the abutting MEF2 and PU.1 sites in a DNA-looping interaction.

  title={The promoter of the Immunoglobulin J Chain gene receives its authentic enhancer activity through the abutting MEF2 and PU.1 sites in a DNA-looping interaction.},
  author={Jung-Hyun Lim and Hong-Gi Kim and Sung-Kyun Park and C. Kang},
  journal={Journal of molecular biology},
  volume={390 3},
Immunoglobulin J chain (IgJ) promoter had previously been dissected in the context of a heterologous enhancer and/or promoter because its strength was weak and its authentic enhancer was not available at that time. Thus, it has been questioned whether the previous dissection of the IgJ promoter might also be relevant in the context of its authentic enhancer. Now that the authentic IgJ enhancer has been identified, redelineation of the IgJ promoter could be performed in the context of this… Expand
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Crlz1 activates transcription by mobilizing cytoplasmic CBFbeta into the nucleus.
In coimmunop Recipitation and chromatin immunoprecipitation experiments, Crlz1 was found to be bound to the resulting CBF heterodimer in a form of ternary complex and to remain in that ternARY complex even when CBF bound to its target DNA site such as IgJ enhancer. Expand
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Pronounced cohabitation of active immunoglobulin genes from three different chromosomes in transcription factories during maximal antibody synthesis.
To understand the relationships between nuclear organization and gene expression in a model system, we employed three-dimensional imaging and chromatin immunoprecipitation (ChIP)-chromosomeExpand
antibody synthesis different chromosomes in transcription factories during maximal Pronounced cohabitation of active immunoglobulin genes from three Material
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A stage-specific enhancer of immunoglobulin J chain gene is induced by interleukin-2 in a presecretor B cell stage.
Interleukin-2 (IL-2)-induced transcription of the J chain gene was used as a model for analyzing cytokine regulation during B cell development and it was shown that the enhancer is activated at this stage by an IL-2 signal that functions by opening the enhancing chromatin and stimulating STAT5 to bind to a STAT5 element critical for the enhancers induction. Expand
Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element.
An important role is established for PU.1 in the regulation of immunoglobulin J-chain gene expression and new insights are provided into the function(s) of the Ets transcription factors in lymphoid cells. Expand
B Cell-specific Activator Protein Prevents Two Activator Factors from Binding to the Immunoglobulin J Chain Promoter until the Antigen-driven Stages of B Cell Development*
It is not until the levels of BSAP decrease during the antigen-driven stages of B cell development that both USF and B-MEF2 are able to bind to their respective promoter elements and activate J chaintranscription. Expand
A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene.
In a primary immune response a signal from interleukin 2 (IL-2) induces B lymphocytes to express the gene for the IgM joining component, the J chain, and analysis of the J-chain gene 5' flanking region identified a major control region that encodes two adjacent elements: a T-rich sequence (JA) containing a single positive regulatory motif and an A+G-rich sequences (JB) containing overlapping positive and negative regulatory motifs. Expand
Structural and Functional Cross-Talk between a Distant Enhancer and the ɛ-Globin Gene Promoter Shows Interdependence of the Two Elements in Chromatin
The results indicate that the ɛ-globin promoter and HS2 interact both structurally and functionally and that both upstream activators and the basal transcription apparatus contribute to the interaction, indicating that transcription activation and promoter remodeling by a distant enhancer are not separable. Expand
Myocyte Enhancer Factor-related B-MEF2 Is Developmentally Expressed in B Cells and Regulates the Immunoglobulin J Chain Promoter*
It is concluded that a MEF2-related transcriptional factor, B-MEF2, acts as a stage-specific positive regulator of J chain gene expression in the B cell lineage. Expand
Gamma-Globin Gene Promoter Elements Required for Interaction With Globin Enhancers
The results suggest that CCAAT boxes, but not GATA or CACCC binding sites, are required for interaction between the γ-globin promoter and the LCR/5′HS2 and that regulatory elements in addition to the core enhancer may be required for the enhancer to act from a distance. Expand
Immunoglobulin J chain gene from the mouse.
Structural properties indicate that transcription of the J chain gene is initiated by changes in chromatin conformation, probably involving a J chain-specific DNA-binding factor. Expand
The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP.
Complete correlation between the expression pattern of the CD19 gene and the B-cell-specific transcription factor BSAP is demonstrated in a large panel of B-lymphoid cell lines and strongly implicates BSAP in the regulation of theCD19 gene. Expand
The HSS3/4 enhancer of Crlz1-IgJ locus is another target of EBF in the pre-B cell stage of B cell development.
It is shown that the protein causing the pre-B cell-specific in vivo footprint on a sequence similar to the EBF-binding consensus is truly EBF as judged by EMSA using various oligo-DNA competitors and anti-EBF antibodies. Expand