The profiling and identification of the metabolites of (+)-catechin and study on their distribution in rats by HPLC-DAD-ESI-IT-TOF-MS(n) technique.

  title={The profiling and identification of the metabolites of (+)-catechin and study on their distribution in rats by HPLC-DAD-ESI-IT-TOF-MS(n) technique.},
  author={Jing Liang and Feng Xu and Ya-Zhou Zhang and Xin-yu Zang and Dan Wang and Ming-Ying Shang and Xuan Wang and De Hua Chui and Shao-Qing Cai},
  journal={Biomedical chromatography : BMC},
  volume={28 3},
(+)-Catechin, a potential beneficial compound to human health, is widely distributed in plants and foods. A high-performance liquid chromatography with diode array detector and combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry method was applied to profile and identify the metabolites of (+)-catechin in rats and to study the distribution of these metabolites in rat organs for the first time. In total, 51 phase II metabolites (44 new) and three phase I… 

Figures and Tables from this paper

Profiling and identification of (-)-epicatechin metabolites in rats using ultra-high performance liquid chromatography coupled with linear trap-Orbitrap mass spectrometer.

An efficient strategy based on ultra-high performance liquid chromatography coupled with a linear ion trap-Orbitrap mass spectrometer was developed to profile and characterize EC metabolites in rat urine, faeces, plasma, and various tissues, demonstrating that liver and kidney were the most important organs for EC and its metabolites elimination.

Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique

The results of metabolite distributions in organs indicated that the conjugated reaction of free procyanidin B2 mainly occurred in liver and diversified metabolites forms were observed in small intestine, and a possible metabolic pathway was proposed.

Characterization and identification of in vitro metabolites of (-)-epicatechin using ultra-high performance liquid chromatography-mass spectrometry

This is the first report on metabolites of (-)-epicatechin in MFRHs, and it is helpful in gaining deeper insight into the metabolism in vivo, and the results will also provide guidance in research on the pharmacokinetics of new drugs.

Detection of 191 Taxifolin Metabolites and Their Distribution in Rats Using HPLC-ESI-IT-TOF-MSn

This is the first report of the in vivo biotransformation of a single compound into more than 100 metabolites and indicates that taxifolin is bioactive not only in the parent form, but also through its metabolites.

Metabolic profiles of 11,13α-dihydroixerin Z in rats using high performance liquid chromatography-LTQ-Orbitrap mass spectrometry

Hydroxylation, hydrolysis, methylation, cysteine conjugation, glutathione (GSH) conjugated, sulfate conjugations, N-acetylcysteineconjugation, and glucuronidation were found to comprise the major metabolic reactions of DIZ in rats.

Exploring the In Vivo Existence Forms (23 Original Constituents and 147 Metabolites) of Astragali Radix Total Flavonoids and Their Distributions in Rats Using HPLC-DAD-ESI-IT-TOF-MSn

The original constituents and metabolites existing in vivo may be material basis for ARTF efficacy, and these findings are helpful for further clarifying the effective forms of ARTF.

The Profiling and Identification of the Absorbed Constituents and Metabolites of Guizhi Decoction in Rat Plasma and Urine by Rapid Resolution Liquid Chromatography Combined with Quadrupole-Time-of-Flight Mass Spectrometry

A method incorporating rapid resolution liquid chromatography (RRLC) with quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) was used to identify ingredients after oral administration of Guizhi decoction and showed that the predominant metabolic pathways of (epi) catechin and gallic acid were sulfation, methylation, glucuronidation and dehydroxylation.

Multi-analysis strategy for metabolism of Andrographis paniculata in rat using liquid chromatography/quadrupole time-of-flight mass spectrometry.

High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was applied to analysis and characterization of AP metabolism in rat urine and feces samples after oral administration of ethanol extract, suggesting that some ingredients' metabolic processes were not unique.

Inhibitory Effects of Green Tea Polyphenols on Microbial Metabolism of Aromatic Amino Acids in Humans Revealed by Metabolomic Analysis

Interestingly, GTP decreased the levels of microbial metabolites of aromatic amino acids (AAA), including indoxyl sulfate, phenylacetylglutamine, and hippuric acid, in urine, however, it did not affect the Levels of AAA, as well as other microbial metabolites, including short-chain fatty acids and secondary bile acids, in feces.



Identification of the major antioxidative metabolites in biological fluids of the rat with ingested (+)-catechin and (-)-epicatechin.

It is concluded that (+)-catechin 5-O-beta-glucuronide and (-)-epicatechin 5 -O- beta-glUCuronide are the biologically active in vivo structures of the ingested polyphenolic antioxidants.

Determination of catechins and catechin gallates in tissues by liquid chromatography with coulometric array detection and selective solid phase extraction.

Studies on flavonoid metabolism. Metabolism of (+)-catechin in the guinea pig.

Degradation of (+)-catechin in the guinea pig is effected at least in part by the gut microflora and is suppressed by aureomycin plus phthaloyl-sulphathiazole.

Procyanidin dimers are metabolized by human microbiota with 2-(3,4-dihydroxyphenyl)acetic acid and 5-(3,4-dihydroxyphenyl)-gamma-valerolactone as the major metabolites.

In vitro fermentation of purified PC dimers with human microbiota revealed two main metabolites, which were responsible for the health effects of chocolate and wine and a degradation pathway, partly different from that of monomeric flavan-3-ols, is proposed.

Profile of plasma and urine metabolites after the intake of almond [Prunus dulcis (Mill.) D.A. Webb] polyphenols in humans.

This study constitutes the most complete report of the absorption of almond skin polyphenols in humans and indicates the important role of intestinal bacteria in the metabolism of highly polymerized almond skinpolyphenols.

The microbial metabolism of (+)-catechin to two novel diarylpropan-2-ol metabolites in vitro.

  • G. GroenewoudH. Hundt
  • Chemistry, Biology
    Xenobiotica; the fate of foreign compounds in biological systems
  • 1984
The metabolism of (+)-catechin by rat-caecal microflora in vitro was investigated and it was found to undergo partial heterocyclic ring cleavage of two novel diarylpropan-2-ol metabolites.

The metabolism and excretion of 3-palmitoyl-(+)-catechin in the rat.

Study in vitro showed that intestinal micro-organisms were unable to metabolize 3-palmitoyl-(+)-catechin and the compound did, however, undergo deesterification in plasma and also in liver-perfusion studies, and was considered to arise from biliary metabolites not containing the ester linkage.

ESI Fragmentation Studies of Four Tea Catechins

The fragmentation pathways of tea catechins(two group stereoisomers) were studied via the ion-trap time-of-flight(IT-TOF) mass spectrometer with the advantages of high mass accuracy,high