The proapoptotic BH3-only protein BAD transduces cell death signals independently of its interaction with Bcl-2

@article{Adachi2002ThePB,
  title={The proapoptotic BH3-only protein BAD transduces cell death signals independently of its interaction with Bcl-2},
  author={Maki Adachi and Kenichi Imai},
  journal={Cell Death and Differentiation},
  year={2002},
  volume={9},
  pages={1240-1247}
}
The BH3-only protein BAD binds to Bcl-2 family proteins through its BH3 domain. Recent studies suggest that BAD binds to both Bcl-2 and Bcl-XL, however mediates its pro-apoptotic functions through inhibition of Bcl-XL, but not Bcl-2. In this paper we addressed this issue using a BAD mutant within the BH3 domain, by substitution of Asp 119 with Gly (BADD119G), which selectively abrogates an ability to interact with Bcl-2. Confocal microscopy revealed that mutation of BAD at D119 does not affect… CONTINUE READING