The prion-like protein Doppel enhances ovine spermatozoa fertilizing ability.

@article{Pimenta2012ThePP,
  title={The prion-like protein Doppel enhances ovine spermatozoa fertilizing ability.},
  author={Jorge Leit{\~a}o Pimenta and Fernanda Moura Vargas Dias and Claudia Coelho Marques and Maria C{\'e}u Baptista and Marco Vasques and Ant{\'o}nio E. M. Horta and Jo{\~a}o Pedro Barbas and Robert Soares and Patr{\'i}cia dos Santos Mesquita and Elsa Cabrita and Carlos Mga Fontes and Jos{\'e} A. M. Prates and Rafael Morais Pereira},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2012},
  volume={47 2},
  pages={
          196-202
        }
}
The function of prion-like protein Doppel was suggested to be related to male fertility. In this study, the importance of ovine Doppel polypeptide on spermatozoa capacitation and fertilization was evaluated. After refolding, recombinant Doppel (rDpl) was supplemented with different concentrations (40, 80 or 190 ng/ml) to ovine spermatozoa during the capacitation process. In experiment 1, post-thawed ovine spermatozoa were incubated with different concentrations of rDpl during 1 h for swim-up… CONTINUE READING
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