The primary structure of the N-terminal region of mature alkaline phosphatase is critical for secretion and function of the enzyme.

@article{Kononova2000ThePS,
  title={The primary structure of the N-terminal region of mature alkaline phosphatase is critical for secretion and function of the enzyme.},
  author={Svetlana V Kononova and Sergey N. Zolov and Vladimir I. Krupyanko and Marina A. Nesmeyanova},
  journal={Biochemistry. Biokhimiia},
  year={2000},
  volume={65 9},
  pages={1075-81}
}
The export signal has been assumed to be localized not only in the signal peptide of a secreted protein precursor, but also in the N-terminal region of the mature polypeptide chain. Mutant alkaline phosphatases with amino acid substitutions of two positively charged residues (Lys or Arg) in this region at different distances from the signal peptide have been studied to test this assumption. The efficiency of secretion has been shown to decrease in mutant proteins with amino acid substitutions… CONTINUE READING

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