The primary structure of coho salmon growth hormone and its cDNA.

@article{Nicoll1987ThePS,
  title={The primary structure of coho salmon growth hormone and its cDNA.},
  author={Charles S. Nicoll and S S Steiny and David S. King and Richard S. Nishioka and Ga{\'e}tan Mayer and Norman L. Eberhardt and J. D. Baxter and Maiko Yamanaka and Judith A. Miller and J. J. Seilhamer},
  journal={General and comparative endocrinology},
  year={1987},
  volume={68 3},
  pages={387-99}
}
Total RNA was extracted from coho salmon growth hormone (sGH) cell regions and used to synthesize double-stranded cDNA, which was inserted into a plasmid vector and used to transform Escherichia coli HB101. The total RNA was also separated according to size by electrophoresis on agarose gels and the fraction that directed the cell-free synthesis of protein in the size range of GHs of other species was isolated and used to screen the transformed colonies of E. coli. A clone containing the… CONTINUE READING