The presence of outer arm fucose residues on the N-glycans of tissue inhibitor of metalloproteinases-1 reduces its activity.

@article{Kim2013ThePO,
  title={The presence of outer arm fucose residues on the N-glycans of tissue inhibitor of metalloproteinases-1 reduces its activity.},
  author={Han Ie Kim and Radka Saldova and Jun Hyoung Park and Young Hun Lee and David J. Harvey and Mark R Wormald and K. Wynne and Giuliano Elia and Hwa-Jung Kim and Pauline M. Rudd and Seung-Taek Lee},
  journal={Journal of proteome research},
  year={2013},
  volume={12 8},
  pages={3547-60}
}
Tissue inhibitor of metalloproteinases-1 (TIMP-1) inhibits matrix metalloproteinases (MMPs) by binding at a 1:1 stoichiometry. Here we have shown the involvement of N-glycosylation in the MMP inhibitory ability of TIMP-1. TIMP-1, purified from HEK 293 cells overexpressing TIMP-1 (293 TIMP-1), showed less binding and inhibitory abilities to MMPs than TIMP-1 purified from fibroblasts or SF9 insect cells infected with TIMP-1 baculovirus. Following deglycosylation of TIMP-1, all forms of TIMP-1… CONTINUE READING