The presence of histamine and acetylcholine in the spleen of the ox and the horse

@article{DaleThePO,
  title={The presence of histamine and acetylcholine in the spleen of the ox and the horse},
  author={Henry Hallett Dale and Harold Ward Dudley},
  journal={The Journal of Physiology},
  volume={68}
}
THE fact that extracts from different animal organs (liver, lung, voluntary muscle) exhibit physiological activities resembling those of histamine and choline, and contain those bases in quantities sufficient to account for these effects, has been demonstrated in previous communications from this Laboratory (1, 2). When due allowance is made for the inevitable losses in the rather difficult processes of separation, the quantities isolated and chemically identified have been sufficient, indeed… 
Parallel Bioassay as a Method of Identifying Acetylcholine
A SURVEY of the methods used to identify the active principle(s) in extracts of nervous tissue possessing cholinomimetic activity reveals that Hosein and his group1 have offered the only serious
Inability of Parallel Bio-assay to recognize Acetylcholine in Mixtures of Substances with Acetylcholine-like Activity
TLDR
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TLDR
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FAILURE OF THE METHOD OF PARALLEL BIOASSAY TO IDENTIFY ACETYLCHOLINE IN MIXTURES OF SUBSTANCES WITH ACETYLCHOLINE-LIKE ACTIVITY.
TLDR
It was concluded that the method of parallel bioassay cannot be used to identify acetylcholine in extracts unless it is shown by other means that acetylCholine is the only active substance present in the material being assayed.
Is any Free Acetylcholine Preformed in Resting Muscles or in the Hertrt?1
TLDR
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History and Basic Properties of the Muscarinic Cholinergic Receptor
TLDR
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Acetylcholine in non‐nervous tissues of some lepidoptera
TLDR
The results described in this communication again raise the question whether ACh and related compounds may have functions in nature in addition to that of a neuro-transmitter substance.
The cardio-active principle in spleen.
A substance has been demonstrated in acetone extracts of freeze-dried spleen which had cardiotonic properties on isolated papillary muscles from the cat and perfused hearts of the cat and guinea-pig.
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