The presence of ATP + ubiquitin-dependent proteinase and multicatalytic proteinase complex in bovine brain

@article{Azaryan1989ThePO,
  title={The presence of ATP + ubiquitin-dependent proteinase and multicatalytic proteinase complex in bovine brain},
  author={Anahit Azaryan and Miriam Banay-Schwartz and Abel Lajtha},
  journal={Neurochemical Research},
  year={1989},
  volume={14},
  pages={995-1001}
}
The presence of two distinct high-molecular-weight proteases with similar pH optima in the weakly alkaline region was shown in cytosol of the bovine brain cortex. They were separated by ammonium sulfate fractionation and each was further purified by DEAE-Sephacel Sephacryl S-300, DEAE-Cibacron Blue 3GA-agarose, heparin-agarose, and Sepharose 6B… CONTINUE READING