The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sections

Abstract

The preparation of a protein A-gold complex (pAg3) using 3 nm gold particles and its application for labelling of intracellular antigens on thin sections is reported. The 3 nm gold particle is the smallest metal particle currently available for cytochemistry and permits a higher resolution of the pAg technique. Furthermore, it can be used in double labelling experiments in conjunction with a pAg complex prepared from 15 nm gold particles. For double labelling, the pAg3 complex must be used for staining of the first antigen since otherwise a non-specific co-labelling of the two pAg complexes results.

DOI: 10.1007/BF01033628

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Cite this paper

@article{Roth1982ThePO, title={The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sections}, author={Jurgen Roth}, journal={The Histochemical Journal}, year={1982}, volume={14}, pages={791-801} }