The preparation and characterization of synaptic vesicles of high purity

@article{Nagy1976ThePA,
  title={The preparation and characterization of synaptic vesicles of high purity},
  author={A Nagy and Richard R. Baker and Steven J. Morris and Victor P. Whittaker},
  journal={Brain Research},
  year={1976},
  volume={109},
  pages={285-309}
}
Very pure preparations of synaptic vesicles have been obtained from guinea pig cerebral cortex and from the electromotor synapses of Torpedo marmorata by density gradient centrifugation in a zonal rotor followed by chromatography on columns of glass beads of controlled pore size. Markers for soluble cytoplasm (lactate dehydrogenase), plasma and endoplasmic membranes membranes (Na-K-ATPase; acetylcholinesterase, NADPH-cytochrome c reductase], mitochondrial membranes [cytochrome oxidase] and… CONTINUE READING

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