The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.

@article{Gasior1979ThePA,
  title={The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.},
  author={E J Gasior and Flor Herrera and Isaac L. Sadnik and Calvin McLaughlin and Kivie Moldave},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 10},
  pages={3965-9}
}
A cell-free protein-synthesizing system has been prepared from Saccharomyces cerevisiae by differential centrifugation of lysed spheroplasts. The preparation, a modified 100,000 x g supernatant fraction, contains ribosomes and monosomes, ribosomal subunits, translation factors, and aminoacyl-tRNA synthetases, but no polysomes. After removal of small amounts of remaining mRNA with micrococcal nuclease, protein synthesis is stringently dependent on the addition of mRNA, as well as amino acids and… CONTINUE READING
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