The predicted amino acid sequence of a centrosphere protein in dividing sea urchin eggs is similar to elongation factor (EF-1 alpha).

@article{Kuriyama1990ThePA,
  title={The predicted amino acid sequence of a centrosphere protein in dividing sea urchin eggs is similar to elongation factor (EF-1 alpha).},
  author={Ryoko Kuriyama and P B Savereide and Philippe Lefebvre and Shamik Dasgupta},
  journal={Journal of cell science},
  year={1990},
  volume={95 ( Pt 2)},
  pages={231-6}
}
Monoclonal antibody (SU5), prepared from isolated mitotic spindles of sea urchin eggs, stained centrospheres preferentially and recognized a 50K (K = 10(3) Mr) polypeptide on immunoblots. Three positive clones were isolated by screening a lambda gt11 cDNA expression library prepared from sea urchin egg mRNA with SU5. One clone containing a 1.8-kb (1 kb = 10… CONTINUE READING