The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.

@article{Niesner2007ThePO,
  title={The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.},
  author={Raluca Aura Niesner and Volker Andresen and Jens Neumann and Heinrich Spiecker and Matthias Gunzer},
  journal={Biophysical journal},
  year={2007},
  volume={93 7},
  pages={2519-29}
}
Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, allowing sample illumination in real time at full laser power. This technology requires charge-coupled device field detection in contrast to… CONTINUE READING

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