The power issue: determination of KB or Ki from IC50. A closer look at the Cheng-Prusoff equation, the Schild plot and related power equations.

@article{Cheng2001ThePI,
  title={The power issue: determination of KB or Ki from IC50. A closer look at the Cheng-Prusoff equation, the Schild plot and related power equations.},
  author={Hsien C Cheng},
  journal={Journal of pharmacological and toxicological methods},
  year={2001},
  volume={46 2},
  pages={61-71}
}
  • Hsien C Cheng
  • Published 2001 in
    Journal of pharmacological and toxicological…
INTRODUCTION The Cheng-Prusoff equation (1973) is often applied to the determination of equilibrium dissociation constant (KB) of a competitive antagonist when the IC50 value is available. The purpose of this study is to illustrate that the slope function (K) of an agonist concentration-response curve is critical to the determination of KB values. METHODS The article describes new equations, which incorporate the slope function, consequently yielding more accurate estimation of KB values for… CONTINUE READING