The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for studies of nuclear architecture.

@article{Markaki2012ThePO,
  title={The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for studies of nuclear architecture.},
  author={Yolanda Markaki and Daniel Smeets and Susanne Fiedler and Volker J. Schmid and Lothar Schermelleh and Thomas Cremer and Marion Cremer},
  journal={BioEssays : news and reviews in molecular, cellular and developmental biology},
  year={2012},
  volume={34 5},
  pages={412-26}
}
Three-dimensional structured illumination microscopy (3D-SIM) has opened up new possibilities to study nuclear architecture at the ultrastructural level down to the ~100 nm range. We present first results and assess the potential using 3D-SIM in combination with 3D fluorescence in situ hybridization (3D-FISH) for the topographical analysis of defined nuclear targets. Our study also deals with the concern that artifacts produced by FISH may counteract the gain in resolution. We address the… CONTINUE READING