The porcine Gpr3 gene: molecular cloning, characterization and expression level in tissues and cumulus–oocyte complexes during in vitro maturation

@article{Zhang2011ThePG,
  title={The porcine Gpr3 gene: molecular cloning, characterization and expression level in tissues and cumulus–oocyte complexes during in vitro maturation},
  author={Baole Zhang and Jian Ding and Ye Li and Jingjing Wang and Yong-yan Zhao and Wei Wang and Shuai Shi and Fulu Dong and Zijing Zhang and Fangxiong Shi and Yinxue Xu},
  journal={Molecular Biology Reports},
  year={2011},
  volume={39},
  pages={5831-5839}
}
G protein-coupled receptor 3 (Gpr3) is a member of G protein-coupled receptor rhodopsin family, which is present throughout the follicle within the ovary and functions as a critical factor for the maintenance of meiotic prophase arrest in oocytes by a Gs protein-mediated pathway. In the current paper, attempts were made to clone and characterize a gene encoding Gpr3 from pigs and investigate its expression pattern in tissues and the whole cumulus–oocyte complexes (COCs) in vitro maturation (IVM… 
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9 Citations
Background Citations
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Methods Citations
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9 Citations

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Citation Type

Sphingosine 1-phosphate acts as an activator for the porcine Gpr3 of constitutively active G protein-coupled receptors
TLDR
Findings suggest the porcine Gpr3, Gpr6, and Gpr12 genes are identified as a subfamily of G protein-coupled receptors, and porcines GPR3 was a constitutively active Gprotein-Coupled receptor.
Immunolocalization and expression pattern of Gpr3 in the ovary and its effect on proliferation of ovarian granulosa cells in pigs.
TLDR
The stage- and cell-specific expression pattern of Gpr3 in the porcine ovary suggested that GPR3 might play an important role during the entire process of follicular development and luteinization.
Characterization of Four Orphan Receptors (GPR3, GPR6, GPR12 and GPR12L) in Chickens and Ducks and Regulation of GPR12 Expression in Ovarian Granulosa Cells by Progesterone
TLDR
The data reveal the structure, function, and expression of GPR3, GPR6, G PR12, and GPR12L in birds, thus providing the first piece of evidence that GPR 12 expression is upregulated by gonadal steroid (i.e., progesterone) in vertebrates.
FSH Modulates PKAI and GPR3 Activities in Mouse Oocyte of COC in a Gap Junctional Communication (GJC)-Dependent Manner to Initiate Meiotic Resumption
TLDR
It is shown that FSH induces a transient increase in cAMP levels and regulates GJC to control PKAI and GPR3 activities, thereby creating an inhibitory phase after PDE3A and MAPK activities increase, meiosis resumes.
GPR3, GPR6, and GPR12 as novel molecular targets: their biological functions and interaction with cannabidiol
TLDR
Identification of CBD as a new inverse agonist for GPR3, GPR6, and GPR12 provides the initial chemical scaffolds upon which potent and efficacious agents acting on these receptors can be developed, with the goal of developing chemical tools for studying these orphan receptors and ultimately new therapeutic agents.
Structural basis for the binding of a selective inverse agonist AF64394 with the human G-protein coupled receptor 3 (GPR3).
TLDR
The three-dimensional structure and conformational dynamics of GPR3 complexed with the inverse agonist AF64394 are described, finding the Pose 1 as a very stable pose with the least fluctuation during the MD simulation while the Pose 2 underwent a significant fluctuation.
Identification of genes involved in breast cancer and breast cancer stem cells
TLDR
It seems that several genes that are not directly related with hormone metabolism and basic signal transduction pathways might have an important role in relapse and disease progression and, thus, can be targeted for new treatment approaches for breast cancer.
Molecular cloning, expression patterns and functional characterization of Gpr3 in the orange-spotted grouper (Epinephelus coioides)
A study of GPR3, GPR6, of GPR12 as novel molecular targets for cannabidiol.

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