The porcine Gpr3 gene: molecular cloning, characterization and expression level in tissues and cumulus–oocyte complexes during in vitro maturation

@article{Zhang2011ThePG,
  title={The porcine Gpr3 gene: molecular cloning, characterization and expression level in tissues and cumulus–oocyte complexes during in vitro maturation},
  author={Baole Zhang and Jian Ding and Ye Li and Jingjing Wang and Yong-yan Zhao and Wei Wang and Shuai Shi and Fulu Dong and Zijing Zhang and Fangxiong Shi and Yinxue Xu},
  journal={Molecular Biology Reports},
  year={2011},
  volume={39},
  pages={5831-5839}
}
G protein-coupled receptor 3 (Gpr3) is a member of G protein-coupled receptor rhodopsin family, which is present throughout the follicle within the ovary and functions as a critical factor for the maintenance of meiotic prophase arrest in oocytes by a Gs protein-mediated pathway. In the current paper, attempts were made to clone and characterize a gene encoding Gpr3 from pigs and investigate its expression pattern in tissues and the whole cumulus–oocyte complexes (COCs) in vitro maturation (IVM… 
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References

SHOWING 1-10 OF 37 REFERENCES
Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system.
Degenerate oligonucleotide primers designed against known G-protein-coupled receptors were used in polymerase chain reaction amplification to isolate a novel receptor sequence (R4) from a rat
Neural Expression of G Protein-coupled Receptors GPR3, GPR6, and GPR12 Up-regulates Cyclic AMP Levels and Promotes Neurite Outgrowth*
TLDR
Results indicate that expression of the constitutively active GPCRs up-regulates cAMP production in neurons, stimulates neurite outgrowth, and counteracts myelin inhibition.
Expression of Growth Differentiation Factor 9 Messenger RNA in Porcine Growing and Preovulatory Ovarian Follicles1
TLDR
It is concluded that GDF 9 mRNA can be produced by somatic follicular cells in the pig and that cumulus expansion is not preceded or accompanied by an increase in the RA of GDF9 mRNA in any of the tested cell types.
Oocyte-specific expression of Gpr3 is required for the maintenance of meiotic arrest in mouse oocytes.
Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase.
TLDR
The mouse ACCA (mACCA) was therefore recloned by PCR, and expression of mACCA in Cos-7 cells demonstrated that the mouse receptor behaved similarly as a constitutive activator of adenylate cyclase.
GPR3 may not be a potential candidate gene for premature ovarian failure.
Meiotic Arrest in Human Oocytes Is Maintained by a Gs Signaling Pathway1
TLDR
It is demonstrated that human oocytes maintain meiotic arrest prior to the LH surge using a signaling pathway similar to that of rodent oocytes.
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