The poplar root transcriptome: analysis of 7000 expressed sequence tags.

  title={The poplar root transcriptome: analysis of 7000 expressed sequence tags.},
  author={Annegret Kohler and Christine Delaruelle and David T. Martin and Nathalie Encelot and Francis Martin},
  journal={FEBS letters},
  volume={542 1-3},
To date, most poplar expressed sequence tags (ESTs) are from above-ground tissues such as wood, leaf and buds. Here, we present a large-scale production of ESTs from roots of the hybrid cottonwood, Populus trichocarpaxdeltoides. cDNA libraries were generated from the root system of 2-month-old rooted cuttings, and roots of 2.5-month-old cuttings water-stressed for 19 days. Partial sequences obtained from 7013 clones were assembled into 1347 clusters and 3527 singletons. This set of ESTs… CONTINUE READING
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