The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro.

@article{Chapman1998ThePB,
  title={The polypeptide backbone of recombinant human zona pellucida glycoprotein-3 initiates acrosomal exocytosis in human spermatozoa in vitro.},
  author={Nate Chapman and E Kessopoulou and P T Andrews and Duncan D Hornby and Christopher Lowther Robert Barratt},
  journal={The Biochemical journal},
  year={1998},
  volume={330 ( Pt 2)},
  pages={839-45}
}
Human gamete interaction is of fundamental biological importance, yet the molecular interactions between spermatozoa and the zona pellucida are poorly understood. Surprisingly, the role of the polypeptide backbone of zona pellucida glycoprotein 3 (ZP3), the putative ligand for spermatozoa activation, has been largely overlooked. Purified recombinant human ZP3 was expressed in Escherichia coli as a C-terminal fusion to the dimeric glutathione S-transferase (GST) from Schistosoma japonicum and… CONTINUE READING