The polymerase subunit of DNA polymerase III of Escherichia coli. I. Amplification of the dnaE gene product and polymerase activity of the alpha subunit.

@article{Maki1985ThePS,
  title={The polymerase subunit of DNA polymerase III of Escherichia coli. I. Amplification of the dnaE gene product and polymerase activity of the alpha subunit.},
  author={Hisaji Maki and Takashi Horiuchi and Arthur Kornberg},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 24},
  pages={12982-6}
}
The Escherichia coli dnaE gene, which encodes the alpha subunit of DNA polymerase III (pol III) holoenzyme, has been cloned in a plasmid containing the PL promoter of phage lambda and thermally induced to overproduce the alpha subunit. In cells carrying this plasmid (pKH167), the alpha subunit was amplified, after heat induction, to a level of about 0.2% of… CONTINUE READING