The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research.

Abstract

Since publication of the polymerase chain reaction (PCR) technique in 1985 (Saiki et al. Science 1985; 230: 1350-1354), there has been an explosion of reports on its use in medicine and science. We critically review its use both as a diagnostic technique and as a research tool, and show the pathologist how to evaluate PCR data and how to avoid the pitfalls… (More)

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Cite this paper

@article{Wright1990ThePC, title={The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research.}, author={Pat Wright and David Wynford-Thomas}, journal={The Journal of pathology}, year={1990}, volume={162 2}, pages={99-117} }