The polymerase chain reaction: an improved method for the analysis of nucleic acids

@article{Vosberg1989ThePC,
  title={The polymerase chain reaction: an improved method for the analysis of nucleic acids},
  author={H R Vosberg},
  journal={Human Genetics},
  year={1989},
  volume={83},
  pages={1-15}
}
The polymerase chain reaction (PCR) is a method for the selective amplification of DNA or RNA segments of up to 2 kilobasepairs (kb) or more in length. Synthetic oligonucleotides flanking sequences of interest are used in repeated cycles of enzymatic primer extension in opposite and overlapping directions. The essential steps in each cycle are thermal denaturation of double-stranded target molecules, primer annealing to both strands and enzymatic synthesis of DNA. The use of the heat-stable DNA… CONTINUE READING
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