The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase

@article{Nag2007ThePT,
  title={The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase},
  author={Anita Nag and Kazim H Narsinh and Harold G. Martinson},
  journal={Nature Structural &Molecular Biology},
  year={2007},
  volume={14},
  pages={662-669}
}
Eukaryotic poly(A) signals direct mRNA 3′-end processing and also pausing and termination of transcription. We show that pausing and termination require the processing factor CPSF, which binds the AAUAAA hexamer of the mammalian poly(A) signal. Pausing does not require the RNA polymerase II C-terminal domain (CTD) or the cleavage stimulation factor, CstF, that binds the CTD. Pull-down experiments show that CPSF binds, principally through its 30-kDa subunit, to the body of the polymerase. CPSF… CONTINUE READING