The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064.

@article{Kulakova1997ThePH,
  title={The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064.},
  author={Anna N. Kulakova and Michael J. Larkin and Leonid A. Kulakov},
  journal={Microbiology},
  year={1997},
  volume={143 ( Pt 1)},
  pages={109-15}
}
The haloalkane dehalogenase (dhaA) gene from Rhodococcus rhodochrous NCIMB 13064 was cloned and sequenced. Its comparison with the previously studied dhlA gene from Xanthobacter autotrophicus GJ10 did not show homology. However, the amino acid sequences of the products of these genes showed approximately 30% identity and several of the catalytic amino acid residues were conserved in the NCIMB 13,064 dehalogenase. A high level of dhaA expression was demonstrated in Escherichia coli cells and… CONTINUE READING