The plasma membrane of Saccharomyces cerevisiae. Isolation and some properties.

@article{Santos1978ThePM,
  title={The plasma membrane of Saccharomyces cerevisiae. Isolation and some properties.},
  author={Eugenio Santos and Julio R. Villanueva and R. Sentandreu},
  journal={Biochimica et biophysica acta},
  year={1978},
  volume={508 1},
  pages={39-54}
}
The isolation of Saccharomyces cerevisiae plasma membrane was carried out after hypotonic lysis of yeast protoplasts treated with concanavalin A by two independent methods: a, at low speed centrifugation and b, at high speed centrifugation in a density gradient. Several techniques (electron microscopic, enzymic, tagging, etc.) were used to ascertain the degree of purification of the plasma membranes obtained. The low speed centrifugation technique as compared with the other method gave a higher… CONTINUE READING

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