The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.

@article{Seplveda2006ThePM,
  title={The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.},
  author={Maria Rosario Sep{\'u}lveda and Mar{\'i}a Berrocal-Carrillo and Mar{\'i}a Gasset and Ana Teresa Mata},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 1},
  pages={447-53}
}
Here we describe the association of the synaptosomal plasma membrane Ca2+-ATPase (PMCA) from pig cerebellum with cholesterol/sphingomyelin-rich membrane domains (rafts). The PMCA4 was localized exclusively in rafts prepared by flotation in Nycodenz density gradients of ice-cold Brij 96 extracts. This was corroborated by its colocalization with the raft markers cholesterol, ganglioside GM1, and PrP(C). The remaining PMCA isoforms were found in the detergent-soluble fractions, with the majority… CONTINUE READING