The physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (Lolium perenne).

@article{Pudney2003ThePC,
  title={The physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (Lolium perenne).},
  author={Paul D. A. Pudney and Sarah L Buckley and Christopher M. Sidebottom and Sarah Twigg and M. J. Sevilla and Christopher M. Holt and David Roper and J H Telford and ALASTAIR J. MCARTHUR and Peter Lillford},
  journal={Archives of biochemistry and biophysics},
  year={2003},
  volume={410 2},
  pages={238-45}
}
We have characterized a cold-induced, boiling stable antifreeze protein. This highly active ice recrystallization inhibition protein shows a much lower thermal hysteresis effect and displays binding behavior that is uncharacteristic of any AFP from fish or insects. Ice-binding studies show it binds to the (1 0 1 0) plane of ice and FTIR studies reveal that it has an unusual type of highly beta-sheeted secondary structure. Ice-binding studies of both glycosylated and nonglycosylated expressed… CONTINUE READING

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