The physical state of the negative strand of hepatitis C virus RNA in serum of patients with chronic hepatitis C.

@article{Shindo1994ThePS,
  title={The physical state of the negative strand of hepatitis C virus RNA in serum of patients with chronic hepatitis C.},
  author={Michiko Shindo and Adrian M Di Bisceglie and Toshitaka Akatsuka and T L Fong and L. M. Scaglione and M. A. Donets and Jay H. Hoofnagle and Stephen M. Feinstone},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 18},
  pages={8719-23}
}
Negative strands of the hepatitis C virus (HCV) genome (a positive-stranded RNA virus) have been found in a nuclease-resistant form in the serum of patients with HCV infections. We determined whether a complete negative-strand copy is present in the serum, whether the negative strand is particle-associated, and finally, whether it is virion-associated and encapsidated like the positive (genomic) strand. Isopyknic sucrose and cesium chloride density ultracentrifugation followed by a strand… CONTINUE READING