The photochemical cycle of bacteriorhodopsin.

  title={The photochemical cycle of bacteriorhodopsin.},
  author={Richard H. Lozier and Werner Niederberger},
  journal={Federation proceedings},
  volume={36 6},
The reaction cycle of bacteriorhodopsin in the purple membrane isolated from Halobacterium halobium has been studied by optical absorption spectroscopy using low-temperature and flash kinetic techniques. After absorption of light, bacteriohodopsin passes through at least five distinct intermediates. The temperature and pH dependence of the absorbance changes suggests that branch points and/or reversible steps exist in this cycle. Flash spectroscopy in the presence of a pH-indicating dye shows… CONTINUE READING