The phosphorylation of coated membrane proteins in intact neurons

@article{Keen1986ThePO,
  title={The phosphorylation of coated membrane proteins in intact neurons},
  author={James H. Keen and Mark M. Black},
  journal={The Journal of Cell Biology},
  year={1986},
  volume={102},
  pages={1325 - 1333}
}
To complement studies that have demonstrated the prominent phosphorylation of a 50-kD coated vesicle polypeptide in vitro, we have evaluated the phosphorylation of coated membrane proteins in intact cells. A co-assembly assay has been devised in which extracts of cultured rat sympathetic neurons labeled with [32P]-Pi were combined with unlabeled carrier bovine brain coat proteins and reassembled coat structures were isolated by gradient centrifugation. Two groups of phosphorylated polypeptides… CONTINUE READING
Highly Cited
This paper has 46 citations. REVIEW CITATIONS