The phosphoCTD-interacting domain of Topoisomerase I.

  title={The phosphoCTD-interacting domain of Topoisomerase I.},
  author={Jianhong Wu and Hemali P. Phatnani and Tao-shih Hsieh and Arno L. Greenleaf},
  journal={Biochemical and biophysical research communications},
  volume={397 1},
The N-terminal domain (NTD) of Drosophila melanogaster (Dm) Topoisomerase I has been shown to bind to RNA polymerase II, but the domain of RNAPII with which it interacts is not known. Using bacterially-expressed fusion proteins carrying all or half of the NTDs of Dm and human (Homo sapiens, Hs) Topo I, we demonstrate that the N-terminal half of each NTD binds directly to the hyperphosphorylated C-terminal repeat domain (phosphoCTD) of the largest RNAPII subunit, Rpb1. Thus, the amino terminal… CONTINUE READING

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