The phosphatase activity of mammalian polynucleotide kinase takes precedence over its kinase activity in repair of single strand breaks

@article{Dobson2006ThePA,
  title={The phosphatase activity of mammalian polynucleotide kinase takes precedence over its kinase activity in repair of single strand breaks},
  author={Caroline J. Dobson and Sarah L. Allinson},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={2230 - 2237}
}
The dual function mammalian DNA repair enzyme, polynucleotide kinase (PNK), facilitates strand break repair through catalysis of 5'-hydroxyl phosphorylation and 3'-phosphate dephosphorylation. We have examined the relative activities of the kinase and phosphatase functions of PNK using a novel assay, which allows the simultaneous characterization of both activities in processing nicks and gaps containing both 3'-phosphate and 5'-hydroxyl. Under multiple turnover conditions the phosphatase… CONTINUE READING